ecm1 af3937  (R&D Systems)

Journal: Animal Cells and Systems

Article Title: ECM1 is associated with endocrine resistance in ER + breast cancers

doi: 10.1080/19768354.2022.2083235

Figure Lengend Snippet: ECM1 is overexpressed in luminal types of breast cancer. (A) Frequency of ECM1 copy number in CPTAC, METABRIC, TCGA or INSERM breast cancers. (B) Alteration frequency of ECM1 by PAM50 molecular subtype in TCGA (left panel) and METABRIC (right panel) breast cancers. (C and D) Level of ECM1 mRNA by PAM50 subtypes in TCGA (C) and METABRIC (D) breast cancers. Tables contain p values for the corresponding comparison.

Article Snippet: ECM1 (Cat#AF3937) and actin (Cat#4967) antibodies were purchased from R&D systems and Cell Signaling Technology, respectively.

Techniques: Comparison

Journal: Animal Cells and Systems

Article Title: ECM1 is associated with endocrine resistance in ER + breast cancers

doi: 10.1080/19768354.2022.2083235

Figure Lengend Snippet: ECM1 is associated with poor outcomes of luminal B-type breast cancers. (A) OS and RFS of ER + breast cancer patients with low or high ECM1 mRNA levels by the autoselect best cutoff in the Kaplan-Meier Plotter. (B) OS and RFS of luminal A breast cancer patients with low or high ECM1 mRNA levels by the autoselect best cutoff in the Kaplan-Meier Plotter. (C) OS and RFS of luminal B breast cancer patients with low or high ECM1 mRNA levels by the autoselect best cutoff in the Kaplan-Meier Plotter. (D) OS and RFS of luminal B breast cancer patients who were treated with hormone therapies, with low or high ECM1 mRNA levels. (E), OS and RFS of METABRIC breast cancer patients with low or high ECM1 mRNA levels. (F) Relative expression of ECM1 in post-treatment ER + tumors compared to pre-treatment tumors.

Article Snippet: ECM1 (Cat#AF3937) and actin (Cat#4967) antibodies were purchased from R&D systems and Cell Signaling Technology, respectively.

Techniques: Expressing

Journal: Animal Cells and Systems

Article Title: ECM1 is associated with endocrine resistance in ER + breast cancers

doi: 10.1080/19768354.2022.2083235

Figure Lengend Snippet: ECM1 is highly expressed in ER + breast cancer cells with acquired endocrine resistance. (A) Complementary DNA (cDNA) was synthesized by mRNA extracted from CAMA1, MDA-MB-361, and T47D cells grown ± 1 nM E2 for 7 days. cDNA was subjected to real-time quantitative PCR (RT-qPCR) B and C, Levels of ECM1 mRNA (B) and protein (C) in CAMA1 LTED and MDA-MB-361 LTED cells in the absence of E2.

Article Snippet: ECM1 (Cat#AF3937) and actin (Cat#4967) antibodies were purchased from R&D systems and Cell Signaling Technology, respectively.

Techniques: Synthesized, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Animal Cells and Systems

Article Title: ECM1 is associated with endocrine resistance in ER + breast cancers

doi: 10.1080/19768354.2022.2083235

Figure Lengend Snippet: ECM1 ablation inhibits the proliferation of endocrine-resistant ER + breast cancer cells. (A) mRNA extracted from CAMA1 LTED and MDA-MB-361 LTED cells, transfected with either control siRNA or ECM1 siRNA for 48 h, were subjected to RT-qPCR. (B) the number of cells described in A was counted every 3 days for 6 days. (C) Correlation between levels of ECM1 expression and levels of phosphorylated Src at Y416 residue was assessed in ER + breast cancer cell lines (left panel) and ER- breast cancer cell lines (right panel) using the DepMap dataset.

Article Snippet: ECM1 (Cat#AF3937) and actin (Cat#4967) antibodies were purchased from R&D systems and Cell Signaling Technology, respectively.

Techniques: Transfection, Control, Quantitative RT-PCR, Expressing, Residue

Journal: Biology of reproduction

Article Title: CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation.

doi: 10.1095/biolreprod.107.066480

Figure Lengend Snippet: FIG. 2. Quantitative assessment of candi- date ECM and adhesion molecule mRNA expression in trophoblast cells following chemokine (CX3CL1 and CCL14) treatment. Significant elevation in mRNA expression of (A) CTNNA1 (3.5-fold; *** P , 0.0001) and (B) ECM1 (almost 6-fold; *** P , 0.0001) was observed following CX3CL1 treatment. However, there were no significant differ- ences with CCL14 treatment. There was a significant reduction in (C) SPP1 and (D) ITGA6 (2.5-fold; ** P ¼ 0.0022) expression levels following CX3CL1 treatment; CCL14 did not regulate the expression of these genes. A significant increase in (E) MMP12 (more than 3-fold; ** P ¼ 0.0022) and (F) ITGB5 (greater than 2-fold; ** P ¼ 0.0022) expression was evident after treatment with both rhCX3CL1 (CX3CL1) and CCL14. C, Control (no treatment).

Article Snippet: ECM1 protein localization was examined using an affinity-purified sheep polyconal anti-human ECM1 antibody (AF3937; R & D Systems).

Techniques: Expressing, Control

Journal: Biology of reproduction

Article Title: CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation.

doi: 10.1095/biolreprod.107.066480

Figure Lengend Snippet: FIG. 3. Immunohistochemical localization of SPP1 (A–C), integrin a6 (E–G), ECM1 (I– K), and cytokeratin (D, H, and L) in first- trimester human implantation sites. A) SPP1 staining is localized to the syncytiocytotro- phoblast layer (SCT) and the cells within the cell column (CC). Positive staining was also seen in leukocytes (L; in A and B) in the villous tip and in the decidua, as seen in the leukocytes (arrowhead) at high-power magnification in the panel to the right of B. B) Weak staining was also localized to the decidualized stroma (Dec). C) Immunore- active SPP1 was localized to the vEVTs residing in the perivascular decidua and in the vEVTs breaching the spiral arteriole wall. D) Cytokeratin staining confirming EVT cell identity. E) Integrin a6 protein localized to the syncytium and a subpopu- lation of leukocytes, and (F) to the CC. G) Minimal immunostaining was seen for integrin a6 in the vEVTs surrounding the spiral arteriole. H) Cytokeratin staining confirmed the presence of trophoblast cells. I) ECM1 protein localized to iEVTs and to the surrounding decidualized stromal cell ECM. J) Immunoreactive ECM1 was detect- ed in decidualized stroma but not in non- decidualized stroma. Immunostaining was absent in the endometrial glands (Gl) and vasculature (V). K) ECM1 also localized to vEVTs and (L) subpopulations of tropho- blasts in the SCT and in the adjacent CC (arrows). M) Cytokeratin staining confirmed these cells were trophoblasts. No staining was observed when primary antibody was substituted with IgG (see insets in A–C, E–G, and J). Bar ¼ 125 lm (A); 25 lm (B, F); 250 lm (E, J); 12.5 lm (B, as it applies to the panel on the right, C, D, G, H, I, K, L, and M).

Article Snippet: ECM1 protein localization was examined using an affinity-purified sheep polyconal anti-human ECM1 antibody (AF3937; R & D Systems).

Techniques: Immunohistochemical staining, Staining, Immunostaining

Journal: Biology of reproduction

Article Title: CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation.

doi: 10.1095/biolreprod.107.066480

Figure Lengend Snippet: FIG. 2. Quantitative assessment of candi- date ECM and adhesion molecule mRNA expression in trophoblast cells following chemokine (CX3CL1 and CCL14) treatment. Significant elevation in mRNA expression of (A) CTNNA1 (3.5-fold; *** P , 0.0001) and (B) ECM1 (almost 6-fold; *** P , 0.0001) was observed following CX3CL1 treatment. However, there were no significant differ- ences with CCL14 treatment. There was a significant reduction in (C) SPP1 and (D) ITGA6 (2.5-fold; ** P ¼ 0.0022) expression levels following CX3CL1 treatment; CCL14 did not regulate the expression of these genes. A significant increase in (E) MMP12 (more than 3-fold; ** P ¼ 0.0022) and (F) ITGB5 (greater than 2-fold; ** P ¼ 0.0022) expression was evident after treatment with both rhCX3CL1 (CX3CL1) and CCL14. C, Control (no treatment).

Article Snippet: ECM1 protein localization was examined using an affinity-purified sheep polyconal anti-human ECM1 antibody (AF3937; R & D Systems).

Techniques: Expressing, Control

Journal: Biology of reproduction

Article Title: CX3CL1 and CCL14 regulate extracellular matrix and adhesion molecules in the trophoblast: potential roles in human embryo implantation.

doi: 10.1095/biolreprod.107.066480

Figure Lengend Snippet: FIG. 3. Immunohistochemical localization of SPP1 (A–C), integrin a6 (E–G), ECM1 (I– K), and cytokeratin (D, H, and L) in first- trimester human implantation sites. A) SPP1 staining is localized to the syncytiocytotro- phoblast layer (SCT) and the cells within the cell column (CC). Positive staining was also seen in leukocytes (L; in A and B) in the villous tip and in the decidua, as seen in the leukocytes (arrowhead) at high-power magnification in the panel to the right of B. B) Weak staining was also localized to the decidualized stroma (Dec). C) Immunore- active SPP1 was localized to the vEVTs residing in the perivascular decidua and in the vEVTs breaching the spiral arteriole wall. D) Cytokeratin staining confirming EVT cell identity. E) Integrin a6 protein localized to the syncytium and a subpopu- lation of leukocytes, and (F) to the CC. G) Minimal immunostaining was seen for integrin a6 in the vEVTs surrounding the spiral arteriole. H) Cytokeratin staining confirmed the presence of trophoblast cells. I) ECM1 protein localized to iEVTs and to the surrounding decidualized stromal cell ECM. J) Immunoreactive ECM1 was detect- ed in decidualized stroma but not in non- decidualized stroma. Immunostaining was absent in the endometrial glands (Gl) and vasculature (V). K) ECM1 also localized to vEVTs and (L) subpopulations of tropho- blasts in the SCT and in the adjacent CC (arrows). M) Cytokeratin staining confirmed these cells were trophoblasts. No staining was observed when primary antibody was substituted with IgG (see insets in A–C, E–G, and J). Bar ¼ 125 lm (A); 25 lm (B, F); 250 lm (E, J); 12.5 lm (B, as it applies to the panel on the right, C, D, G, H, I, K, L, and M).

Article Snippet: ECM1 protein localization was examined using an affinity-purified sheep polyconal anti-human ECM1 antibody (AF3937; R & D Systems).

Techniques: Immunohistochemical staining, Staining, Immunostaining